* Lack of Records: Our understanding of languages from that era is limited. Records are patchy, and many languages were never written down.
* Defining "Language": What constitutes a distinct language is sometimes blurry. Dialects, regional variations, and related languages can be difficult to classify.
* Population Fluctuations: Populations were constantly shifting, and some languages may have experienced periods of decline or growth.
However, some possibilities for languages with potentially low speaker counts in 400 BC include:
* Languages spoken by small, isolated tribes: These groups often had unique languages with limited geographic spread.
* Languages on the verge of extinction: Some languages might have been losing speakers due to factors like conquest, migration, or assimilation.
Important Note: It's important to remember that this is just speculation. We can't definitively know which language had the fewest speakers in 400 BC.